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Research Infrastructure - Pompeu Fabra University-Barcelona

 
  • Flow Cytometry Unit UPF – CRG

Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Information is also collected regarding the size and structural complexity of each particle. The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows. The Unit currently hosts five analysers and two sorters, and there is the largest Becton Dickinson site in Spain.


Keywords: 

• Low • Cytometry • Analysers • Sorters • Cells • Lasers • Samples • Data analysis • DNA • Enzymes • Antigens


Key Contact:

Head of the Unit:

Òscar Fornas

https://www.crg.eu/en/programmes-groups/crgupf-flow-cytometry-unit



 

  • Genomics Core Facility UPF


The Genomics Core Facility at the UPF provides a wide variety of methods for DNA and RNA analyses. Available equipment include liquid handling robots to automate pipetting tasks,
capillary sequencers for Sanger sequencing and fragment analysis, DNA quantification and quality control with Qubit, Picogreen and Bioanalyzer, real-time PCR and OpenArray system for absolute and relative quantification of nucleic acids (genotyping and gene expression), and two next-generation sequencing platforms from Illumina: MiSeq, ideal for targeted and small genome sequencing and NextSeq, a highly flexible platform performing a broad range of applications, from targeted resequencing to RNA profiling and whole-exome or genome sequencing.


Keywords:

• Genomics • DNA • RNA • Bioanalyzer • OpenArray • Genotyping • Gene Expression • MiSeq • NextSeq • Cell Sequencing


Key Contact:

Head of the Unit:
Núria Bonet

https://www.upf.edu/web/sct-genomics



 

  • Peptide Synthesis Core Facility


Synthetic peptides are useful tools in many areas of biomedical research, including well-known applications such as immunogens (anti-peptide antibodies, vaccines), affinity capture and
purification ligands, intracellular delivery shuttles, anti-infective therapies, etc. Our facility provides synthetic peptides for these and other purposes to a variety of PRBB and extramural
users in public and private research institutions, hospitals and health centres, and pharma and biotech companies. State-of-the- art equipment allows fast, reliable synthesis of peptides in a broad range of sizes, amounts, purities and presentations (free, coupled to carrier proteins or affinity supports, biotinylated, lipidated, fluoro-labelled, etc.)


Keywords:

• Synthetic Peptides • Immunogens • Vaccines • Peptides • Proteins


Key Contact:

Head of the Unit :
David Andreu

https://www.upf.edu/web/sct-peptide-synthesis



 

  • Proteomics Unit


The Proteomics Unit is a joint effort of Universitat Pompeu Fabra and the Center of Genomic Regulation to create an innovative core facility that provides high quality proteomics services to its final users, by providing proper expertise and advice, and by developing new methods and techniques. The Proteomics Unit provides full service in a variety of proteomics applications including protein quantification, identification of post-translational modifications, and data analysis, among others. In addition to the services provided to the research community, the Proteomics Unit also promotes internal technology-driven research as an essential task to keep the unit at the forefront of the proteomics field.


Keywords:

• Proteomics • Quantification • Data Analysis • Technology


Key Contact:

Head of the Unit:
Eduard Sabidó

https://www.crg.eu/en/programmes-groups/crgupf-proteomics-unit


  • Scientific IT Core Facility


The mission of the SIT is to support researchers in order to provide a service that promotes, drives and improves their research projects; provide the best IT infrastructures and technical knowledge maximizing the use to minimize the cost and collaborate in research projects. They guarantee a quality service for research, swift in solving problems, speed up the results of research projects, proximity to the user and their research and be transparent and fair. The aim is to be a reference of quality in the scientific-technical service and advanced technology in the university and to be a HUB of knowledge and IT resources.


Keywords: 

• Hub • IT Resources • Technical Knowledge • Scientific-technical Service


Key Contact:

Juan Manuel Fuentes

https://www.upf.edu/web/sct-sit